APPARATUS WHICH ARE USED IN BIOTECHNOLOGICAL LABORATORY

ELECTRONIC COLONY COUNTER

INSTRUMENTATION AND WORKING : 

                                                                          A device which used to count the colonies of bacterial cell that growing in a culture medium. It is usually consist of an illuminating transparent sheet or plate dividing into sections of known area . petridishes those containing the culture colonies of bacteria are placed over it and then counting the colonies according to the number within the area viewed.

It contains – auto mark pen, wolffhuegel grid glass plate, 110mm magnifying lens , net weight- 3.5kg, supply – 230v AC 50 HZ .

PRINCIPLE :

                           Electronic colony counter is designed to count the accurate colonies of bacterial and mould cells in a petridish within a few time. The purpose of plate colonies counting is to estimate the number of cells present based on their ability to give rise to colonies under some specific conditions of nutritient medium, temprature and time.

USES:

o   Place the petriplate on the center.

o   Use any pen or just point it to count correctly.

o   For more accuracy adjust the senctivity , lighting , beep.

PRECAUTION:

       I.            While the majority of microrganisms are not pathogenic to human and have never been shown to cause illness , under unusual circumstances a few microorganism that are normally pathogenic can act as pathogenic .

     II.         

   All                                                   material , media , tubes , plates, loops, needles, pipettes and other item used for culturing microorganisms should be sterilized by autoclaving .

  III.                                                            use a disinfectant , bleach or 70% ethanol solution, tp wipe down branches and work areas both before and after working with cultures.

  IV.                                                                                                                           Use a disinfectant to clean your hands before and after working with microrganism . nondisinfectant soap will remove surface bacteria and can be used if disinfectant soapin not availav[ble gloves may be use be as protectant.

                                                                                                                                            Use pipette bulbs or pipetting device for the aspiration and disapearing of liquid medium .

  VI.                                                                                                                               Do not eat and drink in the lab because there are many bacterias which are not beneficial for our health.

VII.                                                                                                                              Label everything clearly.

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