UV transilluminator

              UV TRANS ILLUMINATOR

PRINCIPLE: 

                          uv. Transilluminator are used in molecular biology labs to view DNA[oRNA]  that are separated by electrphoresis through an agarose gel. Then added a flouroscent dye which binds with nucleic acid . that’s dye stained  gel to a UVB light source to became visible and fluoresce the DNA segments.

INSTRUMENTATION :                                                                                                                              at the time of electrophoresis , a flourescent dye is used to stained agarose gel which binds with nucleic acids . exposing the stained gel to a UVB light source which causes DNA segments to became visible and flouresce.

PRECAUTIONS: 

1. because EtBr [ ethydium bromide ] isa toxic chemical substance so it has been used safely and handled very carefully.
2. when we want to avoid UVB  as a light source , we can recommended the blue light LED transilluminator such as the one descrides here.
3. a safety lid for viewing the gel which comes in transilluminator , however , when the lid is not in place, safety glasses must be worn when operating the UVB bulb
4. we recomanded thet gloves to be worn bcz EtBr is a toxic substance which is added into the staining gel.

USES :

·       Place sample on the filter area . it is recommanded to place the gel on a gel- tray to protect the filter surface from cut and scratches.

·       Press On\OFF switch on . the uv tbes within the unit should be glowing.

·       Now switch on the main power.

·       When using a transilluminator with multiple UV wavelength dial the knob to the appropriate wavelength setting.

ADVANTAGES:

provision of UV protecting shield.

Best suitable for viewing fluorescent sationed gel.

Gel viewing filter provides high quality performance by facilitating sharpened gel view.

 

Compact in size and light in weight.

Provision of working in both high and low intenties with an aid of simple awitc system.                 

Share this: