MICROTOME
INSTRUMENTATION:
Microtome use steel , glass and diamond blades
depending upon the the specimen being sliced and the desired thickness of the
section being cut . steel blades are used to prepare sections of animals or
plant tissues for light microscopy histology. Glass knives are used to slice
sections of light microscopy and to slice very thin sections for electron
microscopy. Industrial grade diamond knives are used to slice hard materials
such as bones , teeth and plant matter for both light microscopy and for
electron microscopy gem quality diamond knives are used for slicing thin
sections of electron microscopy.
PRINCIPLE:
microtome is a sectioning instrument that
allows the cutting of extremely thin slices of a material known as section .
microtome are used in microscopy , allowing for the preparation of sample for
observation under transmitted light or electrons radiation . it is a method for
the preparation of thin section for materials such as bones, minerals, and
teeth.
WORKING :
Microtome is a common instrument . this device
operates with a staged rotary action such that the cutting is part of the
rotary motion . in a rotary microtome ,blade is fixed in horizontal position .
through the motion of the sample holder, the sample is cut by the knife
position , at which point the fresh section remains on the knifes , at the
highest point of the rotary motion , the sample holders is advanced by the same
thickness as the section that is to be made , allowing for the next section to
be made.
The
flywheel is many microtomes can be operated by hands . this has the advantages
that clean cut be made , as the
relatively large mass of the fly wheel prevents the sample from being
stopped during the sample cut. It cuts thickness between 1 and 60 micron meter.
For hard material , its cits a semi thin section with a thickness of as low as
.5 micron meter.
USES:
1.
Setting up
the rotary microtome .
2.
Put the
prepared blocks of wax having tissue or organ .
3.
Adjust the
sample and rotate the rotor.
4.
The
thickness of slides can be adjusted.
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