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शनिवार, 20 अगस्त 2016

anther culture and haploid plant production

   
      ANTHER CULTURE AND HAPLOID PLANT PRODUCTION

All cells are having chromosome which are having genetic information . Many cells are having single set of chromosome are haploid structures , some are having two set of chromosomes , and others  may be having three or four are triploid and tetraploid respectively.                 

N=  SINGLE SET OF CHROMOSOMES I.e HAPLOID

Gametic  cells  of species are called haploid cells which may be female gametic structure[ovum or egg] or may be male gametic structure[anther in plants and sperms in humans . production of haploid is called haploid production. Haploid cells i.e gametes having a single set of chromosome.

                                                                   When the haploid plant chromosome is doubled , normal chromosome no. of a particular species are obtained are called diploid plants these are homozygous in nature . for obtaining diploid plant from haploid plant using colchicine  . 

haploid plants only in nature generated from unfertilized egg or ovum, but  in laboratories we can produced haploid plant via using male and female gametes . anther and ovary both are the sexual part of any flower of angiosperm plants which are producing pollengrain[ male gamete] and when anthers are cultured in a suitable medium to producing a new plant called anther culture .
           
    GUHA AND MAHESHWARI  developed this technique in [1984] .used Dhatura innoxia  for anther culture .

Haploids are also can be formed by using ovaries and ovules. Firstly this techniques was used to produced haploid of DATURA.  This technique helpful for instantaneous expression of mutations and quick formation of pureline. 

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