MERISTEM CULTURE
MERISTEM CELLS ARE LOCALIZED AT THE TIP OF THE ROOT AND
SHOOT PART OF PLANT BODY ,THESE CELLS ARE ACTIVELY DEVIDEDING CELLS WHICH ARE
UNDIFFERENCIATING CELLS WHICH GIVES TO RISE TO THE PERMANENT TISSUE, MERISTEM
CELLS GROWN INVITRO AFTER REMOVAL FOR OBTAINING VIRAL FREE PLANT .APICAL
MERISTEM AND AXILLARY MERISTEM CELLS ARE GROWN IN VITRO CONDITIION IS KNOWN
MERISTEM CULTURE . ITS INVOLVES THE IMPROVEMENT OF ALREADY EXITING SHOOT CELLS
AND REGENERATION OF ADVENTITIOUS ROOT FROM THE DEVELOPING SHOOT.BUT NOT THE
SHOOT NEW SHOOT MERISTEM . FOR MERISTEM CULTURE USUALLY APICAL SHOOT AND ROOT
TIPS ARE USED . THESE ARE CULTURED IN THE PRESENCE OF CYTOKININ [ BAP].
MERISTEM CULTURRE IS USED IN :
1: PRODUCTION OF VIRUS FREE PLANT LIKE SUGARACANE ,
BANANA, APPLE, POTATO ETC.
2: GERMPLASM CONSERVATION
3: GENERATING TRANSGENIC PLANTS
4: FAST CLONAL GERMENATION.
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