Single
cell culturing [ cell cloning]
Through mitosis single cell derived many cells which are
similar to its parent cell which is called clone and this process of obtaining
clones is called cloning . there are two popular techniques of single cell
culturing are as follows:
·
Bergsmann’s planting technique : mostly
used technique ,cells are suspended into a liquid media on a particular cell
density . sterilized agar medium kept in waterbath at 35c temp. liquid and agar
media both are mixed in equal amount .
1mm thick layer of ca spread on petridish .
then cells are embedded in the agar medium which is
observable under microscope . large colonies are isolated and culture in a new
media and separately.
·
Filter paper raft nurse tissue technique
:
1. Cells are
placed over a piece of filter paper which is placed on the top of callus
culture for many days . the single cell takes its nutrition from its callus and
doing mitosis and increase its number . then colonies are formed these are
isolated and further culture.
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