ENVIRONMENTAL
CONDITIONS FOR ASPECTIC CULTIVATION OF PLANT PART:
1. NUTRIENT
MEDIUM
2. ASEPTIC
CONDITIONS
3. AREATION OF
THE TISSUE
·
NUTRIENT MEDIUM: culture media is depending on the
plant part which used for the culture . in the nutrient medium consisting
inorganic salt sources[ both micro and macro elements ] , carbon and nitrogen
sources [ usually sucrose and ammonium salts respectively] , vitamins [
nicotinic acid , pyridoxine , myoinositol , thiamine] , growth regulators [
Auxin , cytokinin majorly , some other
are also used like gibberellins ,
adscisic acids , polyamines ], other compounds like casein , hydrolysate ,
coconut milk , malt extract, yeast extract, tomato juice etc. may be added.
Plant hormone are also plays an important role in
growth and differentiation of cultured
cells and tissues . the pH of culture medium is should be 5.7 . mostly MS
medium is used for the plant tissue culturing which is developed by murashige
and skoog in 1962. Usually a gelly substance AGAR is used to solidify the culture which gives a
substrate or basement to grow the plant part and plant cells.
·
ASEPTIC CONDITIONS : it contains sugar which helpful
for the growth of microorganisms such as bacteria and fungi . these microbes
are competing with the plant cells and tissues and kill them . so its important
to maintain the aseptic conditions of tissue culture. Complete removal of
microorganisms from any surface is called sterilization.
·
AREATION OF TISSUE : it is also an important aspect of
culture technique. It is taken by occasionally stirring the medium by sterring
or by automatic shaker .Plant part which taken out is called explant
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