Shoot and root culturing
For Shoot culturing cytokinin is used because its promotes
the shoot elongation like BAP, and for root culturing Auxin is used because its
promotes the growth of root like NAA [ naphthalic acetic acid ] when we want to
develop both root and shoot we use the different ratio of the both growth
regulators , excess amount of cytokinin promotes the shoot elongation and
excess amount of auxin promotes the root elongation . and if the ratio of cytokinin
and auxin are equal so root and shoot both are not form it form an unorganized
structure which is called callus .
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