Plant tissue culture
Plant tissue culture is a technique which is used to maintain
and growing plant parts and plant cells ,tissues and organs on an artificial
medium in a suitable environmental condition.
That part is culture is called explants, that is , may be any
part of plant which taken from out side the laboratory and grown under in a
test-tube or in a glass flask in special sterile conditions in a special medium
which is having nutritional substances for the plant.Ability to generate a new
whole plant from any plant part [expalnt] and cells from the apex region of the
shoot and root part is called cellular totipotency.
Gottlieb haberlandt firstly started tissue culture
technique in 1902.
Hormones are used in plant tissue culture are as follows :
·
Auxin [indole -3- acetic acid ]
·
Cytokinins [kinetin, zeatin]
·
Gibberellins [gebbrellic acid ]
·
Abscisic acid [abscisic acid ]
·
Polyamines
For growing plant part environmental
conditions are applying.
A. Nutrient
medium,
B. Aseptic
conditions ,
C. Aeration of
the tissue.
एक टिप्पणी भेजें