Plant
tissue culture methods
Two major methods which are includes in plant tissue culture
are as follows:
1. In vitro
growth – callus and suspension culture .
2. Types of
explants – single cell culture, shoot and root culture , haploid culture , meristem culture, somatic
embryo culture , cybrid production , protoplast culture , embryo culture ,
ovule culture, ovary culture etc.
Callus
and suspension culture : continuous cell
division in explants forms callus . callus is a unorganized structure and mass
which formed by dividatio of explants
cells . for callus formation both auxin [2,4 dichlorophenoxy acetic acid ] and
cytokinin [ BAP benzyl amino purine ] are used both are the growth regulators .
they stimulates the plant growth , it is obtain 2-3 weeks.
Suspension
culture is a culture which is kept in a
culture vessels . cells and tissues both are regularly transfer into new
culture vessel which containing fresh media . this process is called
subculturing . suspension culture is an liquid culture .
These both culture are used for the protoplast isolation , production of
transgenic plant , regeneration of plantlets and biomass production also.
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